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Field Test Antibody Student Modeling Pack Classroom Resources

Instructions

Create a schematic model of an antibody.

  1. Watch the video from the Center for BioMolecular Modeling.
  2. Use the green clips with posts to represent the disulfide bonds between the heavy chains and between the heavy and light chains.
  3. Enhance your model with found materials to indicate the following:
  • N- and C-terminal ends of each chain
  • The 12 immunoglobulin folds that make up this protein
  • The two antigen binding sites
  • The disulfide bonds that connect the four chains together
  • The hinge region of the protein where it is maximally flexible

* Create a legend or model description to explain your enhancements.

Fold the mini toober into a model of a nanobody based on 7KKJ.pdb.

  1. Watch the video.
  2. Use a scale of 1 cm per amino acid.
  3. Use Jmol or another molecular visualization tool to see the 3D shape.
  4. Enhance your model with found materials to indicate the following:
  • N- & C-terminal ends of the protein
  • Arrows on each strand of beta-sheet indicating N to C direction of backbone
  • Identify parallel and antiparallel beta-sheet strands
  • The two cysteine amino acids (Cys22 and Cys96) that form the disulfide bond connecting the two beta-sheets

* Create a legend or model description to explain your enhancements.

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